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| Li,Liang-Mei; Li,Jun; Zhang,Xiu-Ying. |
| Background: The antimicrobial properties and molecular interaction analysis of curcumin and its derivatives against three different strains of Streptococcus pneumoniae (Penicillin-susceptible, Penicillin-intermediate and Penicillin-resistant) are studied. Results: These properties were analyzed based on the measurement of the inhibition zone, minimum inhibitory concentration (MIC), and rate of kill revealed that curcumin monoglucoside, curcumin diglucoside and curcumin possessed strong antimicrobial properties even on the Penicillin-resistant strains. Additionally, the molecular docking simulation analyses against Penicillin Binding Protein of S. pneumoniae also confirm that these compounds docked at the active site of the enzyme. Further, the molecular... |
| Tipo: Journal article |
Palavras-chave: Antimicrobial; Curcumin; MIC; Molecular docking; Penicillin-resistant. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100002 |
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| Cao,Yuanyuan; Yao,Jianming; Li,Jun; Chen,Xiangsong; Wu,Jinyong. |
| To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a ?saddle? shape, and the positive mutation rate was increased to 22.00% at the dose of nitrogen ions 2.0 x 10(15) ions/cm² when the survival rate was 28.60%. By repeated screening, a high lipid producing strain G9 was obtained. The biomass, lipid content and lipid yield of the mutant can reach 40.25 g/L, 71.14% and 28.63 g/L after cultured in a 5L fermenter for 8 days, increasing by 52.81%, 68.82% and 157.93% to those of wild strain, respectively. Analysis results... |
| Tipo: Journal article |
Palavras-chave: Fatty acids; Geotrichum robustum; High lipid producing strain; Ion beam implantation. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600002 |
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| Weng,Yali; Chen,Nian; Han,Yaping; Xing,Yiping; Li,Jun. |
| Severe fever with thrombocytopenia syndrome (SFTS) associated with severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging infectious disease. 12 patients with severe fever with thrombocytopenia syndrome in our study were presented mainly with fever and severe malaise. The clinical manifestations typically became worse on the 6th or 7th day. The average fever time is 9.11 ± 1.54 days. Most of them had multiorgan dysfunction, and part of them had hemophagocytic lymphohistiocytosis histiocytosis (HLH). The characteristic laboratory findings in the early stage were the drop of white blood cells (WBC), platelets (PLT) and serum Ca++, while increase of aspartate amino transferase (AST), creatine kinase (CK), and lactate dehydrogenase (LDH).... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Thrombocytopenia; Bunyaviridae infection; Clinical features; Laboratory characteristics. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100088 |
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| Li,Jun; Wu,Zelong; Yuan,Jun. |
| ABSTRACT The production of Camellia oleifera (oil tea), typically planted in acidic red soils in southern China, is limited by low soil fertility. Agro-farming is one way to promote soil fertility by increasing organic matter and microbial communities. To understand the impact of agro-farming activity on soil fertility, three types of agro-farming, namely, raising laying hens under forest (RLH), cultivating Lolium perenne grass under forest (LPG), and maintenance of native grass (MNG), were employed in an oil tea farm with acidic red soil in Changsha, China. Soil samples were collected from the farm to estimate microbial communities, pH, and total organic carbon (TOC) in different seasons. The results indicated that TOC and temperature were the dominant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oil tea; Season; Soil microbial community; High throughput sequencing. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832019000100413 |
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| Chen,Yong-Hao; Li,Jun; Liu,Li; Liu,Hong-Zhi; Wang,Qiang. |
| A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus equisimilis mutant; Hyaluronic acid; Optimization; Culture medium; Culture conditions. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400040 |
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| Chen,Liang; Xia,Hengchuan; Wang,Yiting; Chen,Keping; Qin,Lvgao; Wang,Bin; Yao,Qin; Li,Jun; He,Yuanqing; Zhao,Ermi. |
| Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2D electrophoresis; Elaphe taeniura; MALDI-TOF mass spectrometry; Protein. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020 |
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